Reporter

Part:BBa_K216015

Designed by: Edinburgh iGEM 2009   Group: iGEM09_Edinburgh   (2009-10-16)


PyeaR with firefly luciferase

This is the nitrate- and nitrite-responsive promoter PyeaR of Escherichia coli with the luciferase of firefly Photinus pyralis (deposited by Slovenia iGEM 2007) with the addition of a strong bacterial ribosome binding site. It should generate light in the presence of nitrate/nitrite and luciferin.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 932


[edit]
Categories
//function/reporter/light
Parameters
None